Application
Sample (30 ug of whole cell lysate) A: HepG2 10% SDS PAGE diluted at 1:10000Application
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using ENO3 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.| Product Name | ENO3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ENO3 (enolase 3 (beta, muscle))This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in skeletal muscle cells in the adult. A switch from alpha enolase to beta enolase occurs in muscle tissue during development in rodents. Mutations in this gene can be associated with metabolic myopathies that may result from decreased stability of the enzyme. Two transcripts have been identified for this gene that differ only in their 5' UTR. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ENO3. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Zebrafish, Xenopus laevis, Pig, Rabbit, Chicken, BovineMouse (97%), Rat (97%), Zebrafish (85%), Xenopus laevis (89%), Pig (97%), Rabbit (97%), Chicken (82%), Bovine (97%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | GSD13 antibody, MSE antibody, ENO3 antibody, beta-enolase antibody, skeletal muscle enolase antibody, muscle-specific enolase antibody, 2-phospho-D-glycerate hydrolyase antibody, "enolase 3 (beta, muscle) antibody" |
|---|---|
| Molecular Weight(MW) | 47 kDa (note) (3) |
| Cellular Localization | Cytoplasm |
Application
Sample (30 ug of whole cell lysate) A: HepG2 10% SDS PAGE diluted at 1:10000
Application
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using ENO3 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
