Western blot analysis of extracts from JK cells (Lane 2), using EPHA4 (Phospho-Tyr596) Antibody. The lane on the left is treated with antigen-specific peptide.
Rabbit Polyclonal antibody to EphA4 (phospho Tyr596)This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2015]
Immunogen
Peptide sequence around phosphorylation site of tyrosine 596 (R-T-Y(p)-V-D) derived from Human EPHA4 .
Modification
p-Tyr596
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
WB
WB: 1:500 - 1:1000:
Species Reactivity
Human
Concentration
1mg/ml (Please refer to the vial label for the specific concentration)
Purification
Affinity purified
Target Information
Gene Synonyms
EPH receptor A4 EPHA4 TYRO1 SEK HEK8
Target
EphA4 (phospho Tyr596)
Tissue Specificity
The antibody detects endogenous levels of EPHA4 only when phosphorylated at tyrosine 596
Application
Application
Western blot analysis of extracts from JK cells (Lane 2), using EPHA4 (Phospho-Tyr596) Antibody. The lane on the left is treated with antigen-specific peptide.
Application Notes
WB: 1:500 - 1:1000:
Additional Information
Form
Liquid
Storage Instructions
Store at -20˚C/1 year
Storage Buffer
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.