Application
Western blot analysis of EPHA4 (arrow) using EphA4 Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EPHA4 gene (Lane 2).Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary EphA4 Antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. BC = breast carcinomaApplication
Western blot analysis of anti-EphA4 Pab in NCI-H460 cell lysate. EphA4 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.| Product Name | EphA4 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to EphA4Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The tyrosine kinase (TK) group is mainly involved in the regulation of cell-cell interactions such as differentiation, adhesion, motility and death. There are currently about 90 TK genes sequenced, 58 are of receptor protein TK (e.g. EGFR, EPH, FGFR, PDGFR, TRK, and VEGFR families), and 32 of cytosolic TK (e.g. ABL, FAK, JAK, and SRC families). |
| Immunogen | Synthetic peptide conjugated to KLH, corresponding to amino acids 890-904 of Human Eph A4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| ELISA: Use at a dilution of 1/1000. WB: Use at a dilution of 1/100 - 1/500. Detects a band of approximately 108 kDa (predicted molecular weight: 118 kDa). Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Purification | Protein G |
| Gene Synonyms | EPHA4 2043 P54764 TYRO1 HEK8 602188 SEK |
|---|---|
| Target | EphA4 |
| Cellular Localization | Cell Membrane |
Application
Western blot analysis of EPHA4 (arrow) using EphA4 Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EPHA4 gene (Lane 2).
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary EphA4 Antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. BC = breast carcinoma
Application
Western blot analysis of anti-EphA4 Pab in NCI-H460 cell lysate. EphA4 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.| Positive Control | NCI-H460 cell lysate |
|---|---|
| Application Notes | ELISA: Use at a dilution of 1/1000. WB: Use at a dilution of 1/100 - 1/500. Detects a band of approximately 108 kDa (predicted molecular weight: 118 kDa). Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.09% Sodium Azide; Constituents: PBS |
