Application
EPHX1 antibody detects EPHX1 protein at cytosol on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. EPHX1 antibody dilution: 1:500.Application
Sample (30 ug of whole cell lysate) A: Hela B: Hep G2 10% SDS PAGE diluted at 1:1000Application
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using EPHX1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.Application
Immunohistochemical analysis of paraffin-embedded SG xenograft, using Epoxide hydrolase 1 antibody at 1:500 dilution.| Product Name | EPHX1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to EPHX1 (epoxide hydrolase 1, microsomal (xenobiotic))Epoxide hydrolase is a critical biotransformation enzyme that converts epoxides from the degradation of aromatic compounds to trans-dihydrodiols which can be conjugated and excreted from the body. Epoxide hydrolase functions in both the activation and detoxification of epoxides. Mutations in this gene cause preeclampsia, epoxide hydrolase deficiency or increased epoxide hydrolase activity. Alternatively spliced transcript variants encoding the same protein have been found for this gene. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human EPHX1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Pig, Rabbit, Rhesus Monkey, BovineRat (86%), Pig (88%), Rabbit (81%), Rhesus Monkey (96%), Bovine (88%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | EPHX antibody EPOX antibody MEH antibody EPHX1 antibody epoxide hydrolase 1 antibody epoxide hydratase antibody "epoxide hydrolase 1 microsomal (xenobiotic) antibody" |
|---|---|
| Molecular Weight(MW) | 53 kDa (note) (3) |
| Cellular Localization | Microsome membrane; Single-pass membrane protein , Endoplasmic reticulum membrane; Single-pass membrane protein |
Application
EPHX1 antibody detects EPHX1 protein at cytosol on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. EPHX1 antibody dilution: 1:500.
Application
Sample (30 ug of whole cell lysate) A: Hela B: Hep G2 10% SDS PAGE diluted at 1:1000
Application
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using EPHX1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.
Application
Immunohistochemical analysis of paraffin-embedded SG xenograft, using Epoxide hydrolase 1 antibody at 1:500 dilution.| Positive Control | 293T , HeLa , HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
