Application
Erythropoietin was detected in immersion fixed paraffin-embedded sections of human kidney cancer tissue using Human Erythropoietin Polyclonal Antibody at 10 µg/mL overnight at 4 °C (brown) and counterstained with hematoxylin (blue). Lower image shows no staining because no primary antibody was used.| Product Name | EPO antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to EPOErythropoietin is a humoral factor that stimulates erythropoiesis. EPO has four introns and five exons and contains 165 amino acids. EPO induces growth and differentiation of burst forming units and colony forming units into mature red blood cells. Human erythropoietin stimulates growth of rodent bone marrow cells. Recombinant erythropoietin is produced in CHO cells by a DNA sequence encoding the human EPO protein. |
| Immunogen | Recombinant full length protein (Human), expressed in CHO cells. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PWB |
| I-ELISA: Use at a concentration of 1 μg/ml. Detects EPO at 100 ng/lane under both reducing and nonreducing conditions WB: Use at a concentration of 2 μg/ml. Detects a band of 3 ng/well of EPO (approximately 18 kDa). Endotoxin:: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Protein A |
| Gene Synonyms | 133170 MGC138142 EP EPO P01588 2056 |
|---|---|
| Target | EPO |
| Cellular Localization | Secreted |
Application
Erythropoietin was detected in immersion fixed paraffin-embedded sections of human kidney cancer tissue using Human Erythropoietin Polyclonal Antibody at 10 µg/mL overnight at 4 °C (brown) and counterstained with hematoxylin (blue). Lower image shows no staining because no primary antibody was used.| Positive Control | GTX10652 has been tested for its ability to neutralize the biological activity of EPO on the human cell line, TF1. |
|---|---|
| Application Notes | I-ELISA: Use at a concentration of 1 μg/ml. Detects EPO at 100 ng/lane under both reducing and nonreducing conditions WB: Use at a concentration of 2 μg/ml. Detects a band of 3 ng/well of EPO (approximately 18 kDa). Endotoxin:: |
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20 degrees C or -80 degrees C. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | Phosphate-buffered saline, pH 7.2, no preservative added. |
