Application
ER81 antibody detects ER81 protein at nucleus by immunofluorescent analysis. Sample: C8D30 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ER81 protein stained by ER81 antibody diluted at 1:500. Blue: Hoechst 33342 staining.Application
Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with ER81 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
ER81 antibody detects ER81 protein by western blot analysis. A. 30 µg A431 whole cell lysate/extract B. 30 µg HeLa whole cell lysate/extract 10% SDS-PAGE ER81 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | ER81 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ER81 (ets variant 1) |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ER81. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Xenopus laevis, Chicken, Chimpanzee, BovineXenopus laevis (86%), Chicken (92%), Chimpanzee (100%), Bovine (95%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | ETV1 ets variant 1 |
|---|---|
| Target | ER81 |
| Cellular Localization | Nucleus |
Application
ER81 antibody detects ER81 protein at nucleus by immunofluorescent analysis. Sample: C8D30 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ER81 protein stained by ER81 antibody diluted at 1:500. Blue: Hoechst 33342 staining.
Application
Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with ER81 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
ER81 antibody detects ER81 protein by western blot analysis. A. 30 µg A431 whole cell lysate/extract B. 30 µg HeLa whole cell lysate/extract 10% SDS-PAGE ER81 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | A431 , HeLa , Rat2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
