Application
WB analysis of Jurkat cells using ERG antibody at 5 mg/ml. Lane A: Primary Antibody Lane B: Primary Antibody + Blocking Peptide| Product Name | ERG antibody, Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ERGThis gene encodes a member of the erythroblast transformation-specific (ETS) family of transcriptions factors. All members of this family are key regulators of embryonic development, cell proliferation, differentiation, angiogenesis, inflammation, and apoptosis. The protein encoded by this gene is mainly expressed in the nucleus. It contains an ETS DNA-binding domain and a PNT (pointed) domain which is implicated in the self-association of chimeric oncoproteins. This protein is required for platelet adhesion to the subendothelium, inducing vascular cell remodeling. It also regulates hematopoesis, and the differentiation and maturation of megakaryocytic cells. This gene is involved in chromosomal translocations, resulting in different fusion gene products, such as TMPSSR2-ERG and NDRG1-ERG in prostate cancer, EWS-ERG in Ewing's sarcoma and FUS-ERG in acute myeloid leukemia. More than two dozens of transcript variants generated from combinatorial usage of three alternative promoters and multiple alternative splicing events have been reported, but the full-length nature of many of these variants has not been determined. [provided by RefSeq, Apr 2014] |
| Immunogen | A synthetic peptide corresponding to an Internal region of Human ERG |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | Human |
| Predicted Reactivity | Cow: 100%; Dog: 92%; Guinea Pig: 85%; Horse: 100%; Human: 100%; Mouse: 92%; Rabbit: 92%; Rat: 100% |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Synonyms | ERG P55 ERG3 |
|---|---|
| Target | ERG |
| Molecular Weight(MW) | 55 KDa (note) |
Application
WB analysis of Jurkat cells using ERG antibody at 5 mg/ml. Lane A: Primary Antibody Lane B: Primary Antibody + Blocking Peptide| Positive Control | Jurkat |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. For short-term storage, store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
