Application
A. Western blot analysis on serum starved (ss)A431 cell lysate using anti-ERK1(pT202/pY204) /ERK2(pT185/pY187) RabMAb (cat. #); dilution 1:500Application
B. Immunohistochemical analysis of paraffin-embedded human thyroid gland cancer using anti- ERK1(pT202/pY204) /ERK2(pT185/pY187) RabMAb (cat. # )| Product Name | ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit monoclonal antibody [E337] to ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187)Extracellular signal-regulated kinases Erk1 (p44 MAPK) and Erk2 (p42 MAPK) Serine/Threonine protein kinase are part of a subfamily of mitogen-activated protein kinases that participate in diverse cellular functions, including the regulation of cell growth, differentiation, apoptosis and signal transduction . The simultaneous tyrosine/threonine phosphorylation of ERK1 and ERK2 results in their activation. Although ERK1 and ERK2 resemble one another closely in sequence and are activated by similar pathways, they appear to have different tissue distributions and show differential phosphorylation of potential intracellular substrates . |
| Immunogen | A synthetic phospho-peptide corresponding to residues surrounding Thr202 and Tyr204 of human ERK1 was used as immunogen. |
| Modification | p-Thr185/Tyr187 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | E337 |
| Host Species | Rabbit |
| Tested Applications | FACSICC/IFIHC-PWB |
| For WB: Use at a dilution of 1:1000-10,000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | PRKM3 ERK1 MGC20180 MAPK3 P44MAPK HS44KDAP P44ERK1 HUMKER1A Erk1 (Phospho) Phospho Erk1 |
|---|---|
| Target | ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) |
| Molecular Weight(MW) | 42/44 (note) |
| Tissue Specificity | The antibody only detects ERK1 phosphorylated on Threonine 202 and Tyrosine 204, or ERK2 phosphorylated on Threonine 185 and Tyrosine 187. |
Application
A. Western blot analysis on serum starved (ss)A431 cell lysate using anti-ERK1(pT202/pY204) /ERK2(pT185/pY187) RabMAb (cat. #); dilution 1:500
Application
B. Immunohistochemical analysis of paraffin-embedded human thyroid gland cancer using anti- ERK1(pT202/pY204) /ERK2(pT185/pY187) RabMAb (cat. # )| Application Notes | For WB: Use at a dilution of 1:1000-10,000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 °C. Stable for 12 months from date of receipt. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
