Application
Western blot analysis of Estrogen Receptor alpha (pS118) expression in MCF7 EGF-treated (A); HeLa EGF-treated (B) whole cell lysates.Application
Immunohistochemical analysis of Estrogen Receptor alpha (pS118) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.| Product Name | Estrogen Receptor alpha (phospho Ser118) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Estrogen Receptor alpha (phospho Ser118)This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative promoter usage and alternative splicing result in dozens of transcript variants, but the full-length nature of many of these variants has not been determined. [provided by RefSeq, Mar 2014] |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Estrogen Receptor alpha (pS118). The exact sequence is proprietary. |
| Modification | p-Ser118 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| WB: 1:500 - 1:1000. IHC-P: 1:100 - 1:200: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ESR1 ER NR3A1 ESTRR estrogen receptor 1 Era ESR ESRA |
|---|---|
| Target | Estrogen Receptor alpha (phospho Ser118) |
| Tissue Specificity | Recognizes endogenous levels of Estrogen Receptor alpha (pS118) protein. |
Application
Western blot analysis of Estrogen Receptor alpha (pS118) expression in MCF7 EGF-treated (A); HeLa EGF-treated (B) whole cell lysates.
Application
Immunohistochemical analysis of Estrogen Receptor alpha (pS118) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.| Application Notes | WB: 1:500 - 1:1000. IHC-P: 1:100 - 1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4 °C. Upon delivery aliquot and store at -20 °C for one year. Avoid freeze/thaw cycles. |
| Storage Buffer | Liquid in 0.42% Potassium phosphate; 0.87% Sodium chloride; pH 7.3; 30% glycerol; and 0.01% sodium azide. |
