Application
Immunofluorescence staining of methanol-fixed MCF cells using Estrogen Receptor-a(Ab-118) AntibodyApplication
Western blot analysis of extracts from MCF cells using Estrogen Receptor-a(Ab-118) Antibody and the same antibody preincubated with blocking peptide.Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Estrogen Receptor-a(Ab-118) Antibody (left) or the same antibody preincubated with blocking peptide(right).| Product Name | Estrogen Receptor alpha antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Estrogen Receptor alphaNuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. |
| Immunogen | Peptide sequence around aa.116~120 (Q-L-S-P-F) derived from Human Estrogen Receptor alpha . |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | DKFZp686N23123 ER ESR ESRA Era NR3A1 ESR1 estrogen receptor alpha delta 4* 5 6 7*/654 isoform nuclear receptor subfamily 3 group A member 1 estrogen receptor alpha 3* 4 5 6 7*/822 isoform estrogen nuclear receptor alpha estradiol receptor ER-a |
|---|---|
| Target | Estrogen Receptor alpha |
| Molecular Weight(MW) | 66 (note) |
Application
Immunofluorescence staining of methanol-fixed MCF cells using Estrogen Receptor-a(Ab-118) Antibody
Application
Western blot analysis of extracts from MCF cells using Estrogen Receptor-a(Ab-118) Antibody and the same antibody preincubated with blocking peptide.
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Estrogen Receptor-a(Ab-118) Antibody (left) or the same antibody preincubated with blocking peptide(right).| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
