Application
ICC/IF analysis of MCF-7 cells using Estrogen Receptor alpha antibody [EPR4097] at a dilution of 1:200 (green) and DAPI (blue).Application
WB analysis of (1) MCF-7 and (2) T47-D lysates (20 μg per lane) using Estrogen Receptor alpha antibody [EPR4097] at a dilution of 1:2000.Application
IHC-P analysis of human breast tissue using Estrogen Receptor alpha antibody [EPR4097] at a dilution of 1:250. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. Counterstained with Hematoxylin.| Product Name | Estrogen Receptor alpha antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit monoclonal antibody [EPR4097] to ER alphaEstrogen receptor alpha (ER alpha) is a ligand-activated transcription factor and member of the steroid hormone receptor family. It possesses highly conserved DNA binding and ligand binding domains for hormone binding, exerting a significant role in activating the transcription of certain genes . ER alpha binds to estrogen response elements (ERE) to regulate estrogen-responsive gene expression . Ligand-dependent dimerization and phosphorylation both function to regulate the transcriptional activation of ER alpha . Phosphorylation of serines 104 and 106, located in the N-terminal transcription activation function-1 domain (AF-1), regulates ER alpha activity . It is a prognostic marker for breast cancer because ER alpha is present in the mammary gland . |
| Immunogen | A recombinant human ER alpha protein was used as an immunogen. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | EPR4097 |
| Host Species | Rabbit |
| Tested Applications | FACSICC/IFIHC-PWBChIP |
| ChIP: Use at an assay dependent concentration. Is unsuitable for Flow Cyt. IHC-P: 1/250. ICC/IF: 1/250. WB: 1/1000 - 1/10000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Synonyms | ESR Era ER NR3A1 ESRA DKFZp686N23123 ESR1 |
|---|---|
| Target | ER alpha |
| Molecular Weight(MW) | 66 kDa (note) |
Application
ICC/IF analysis of MCF-7 cells using Estrogen Receptor alpha antibody [EPR4097] at a dilution of 1:200 (green) and DAPI (blue).
Application
WB analysis of (1) MCF-7 and (2) T47-D lysates (20 μg per lane) using Estrogen Receptor alpha antibody [EPR4097] at a dilution of 1:2000.
Application
IHC-P analysis of human breast tissue using Estrogen Receptor alpha antibody [EPR4097] at a dilution of 1:250. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. Counterstained with Hematoxylin.| Positive Control | *BT474 |
|---|---|
| Application Notes | ChIP: Use at an assay dependent concentration. Is unsuitable for Flow Cyt. IHC-P: 1/250. ICC/IF: 1/250. WB: 1/1000 - 1/10000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 °C. Stable for 12 months from date of receipt. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
