Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using Estrogen Receptor alpha antibody (Green) at 1:100 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.Application
Estrogen Receptor alpha antibody [N2], N-term detects Estrogen Receptor alpha protein at nucleus on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. Estrogen Receptor alpha antibody [N2], N-term dilution: 1:500.Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Estrogen Receptor alpha antibody [N2], N-term diluted at 1:1000.| Product Name | Estrogen Receptor alpha antibody , N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Estrogen Receptor alpha (estrogen receptor 1)This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative splicing results in several transcript variants, which differ in their 5' UTRs and use different promoters. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Estrogen Receptor alpha. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Dog, Pig, BovineDog (100%), Pig (100%), Bovine (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | DKFZp686N23123 antibody ER antibody ESR antibody ESRA antibody Era antibody NR3A1 antibody ESR1 antibody "estrogen receptor alpha delta 4* 5 6 7*/654 isoform antibody" nuclear receptor subfamily 3 group A member 1 antibody "estrogen receptor alpha |
|---|---|
| Molecular Weight(MW) | 66 kDa (note) (6) |
| Cellular Localization | Nucleus |
Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using Estrogen Receptor alpha antibody (Green) at 1:100 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.
Application
Estrogen Receptor alpha antibody [N2], N-term detects Estrogen Receptor alpha protein at nucleus on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. Estrogen Receptor alpha antibody [N2], N-term dilution: 1:500.
Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Estrogen Receptor alpha antibody [N2], N-term diluted at 1:1000.| Positive Control | ESR1-transfected 293T cells , *MCF7 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
