Application
Immunoprecipitation of ETFA protein from HepG2 whole cell extracts using 5 μg of ETFA antibody . Western blot analysis was performed using ETFA antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.Application
Sample (30 ug of whole cell lysate) A: A431 B: HeLa 10% SDS PAGE diluted at 1:1000Application
ETFA antibody detects ETFA protein by Western blot analysis. A. 30 µg PC-12 whole cell lysate/extract 10 % SDS-PAGE ETFA antibody dilution: 1:1000Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using ETFA antibody at 1:500 dilution.Application
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using ETFA antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.Application
ETFA antibody detects ETFA protein at mitochondria on mouse heart by immunohistochemical analysis. Sample: Paraffin-embedded mouse stomach. ETFA antibody dilution: 1:500.Application
ETFA antibody detects ETFA protein at mitochondria on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. ETFA antibody dilution: 1:500.Application
ETFA antibody detects ETFA protein at mitochondria on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. ETFA antibody dilution: 1:500.| Product Name | ETFA antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ETFA (electron-transfer-flavoprotein, alpha polypeptide)ETFA participates in catalyzing the initial step of the mitochondrial fatty acid beta-oxidation. It shuttles electrons between primary flavoprotein dehydrogenases and the membrane-bound electron transfer flavoprotein ubiquinone oxidoreductase. Defects in electron-transfer-flavoprotein have been implicated in type II glutaricaciduria in which multiple acyl-CoA dehydrogenase deficiencies result in large excretion of glutaric, lactic, ethylmalonic, butyric, isobutyric, 2-methyl-butyric, and isovaleric acids. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ETFA. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Cat, Pig, Chicken, Rhesus Monkey, BovineCat (94%), Pig (94%), Chicken (81%), Rhesus Monkey (97%), Bovine (94%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | EMA antibody GA2 antibody MADD antibody ETFA antibody electron transfer flavoprotein alpha polypeptide antibody electron transfer flavoprotein alpha-subunit antibody electron transfer flavoprotein subunit alpha mitochondrial antibody alpha-ETF ant |
|---|---|
| Molecular Weight(MW) | 35 kDa (note) |
| Cellular Localization | Mitochondrion matrix |
Application
Immunoprecipitation of ETFA protein from HepG2 whole cell extracts using 5 μg of ETFA antibody . Western blot analysis was performed using ETFA antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Application
Sample (30 ug of whole cell lysate) A: A431 B: HeLa 10% SDS PAGE diluted at 1:1000
Application
ETFA antibody detects ETFA protein by Western blot analysis. A. 30 µg PC-12 whole cell lysate/extract 10 % SDS-PAGE ETFA antibody dilution: 1:1000
Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using ETFA antibody at 1:500 dilution.
Application
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using ETFA antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.
Application
ETFA antibody detects ETFA protein at mitochondria on mouse heart by immunohistochemical analysis. Sample: Paraffin-embedded mouse stomach. ETFA antibody dilution: 1:500.
Application
ETFA antibody detects ETFA protein at mitochondria on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. ETFA antibody dilution: 1:500.
Application
ETFA antibody detects ETFA protein at mitochondria on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. ETFA antibody dilution: 1:500.| Positive Control | A431 , HeLa , PC-12 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
