Application
ETFA antibody detects ETFA protein at mitochondria on mouse brain stem by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. ETFA antibody dilution: 1:500.Application
ETFA antibody detects ETFA protein at mitochondria on mouse cervix by immunohistochemical analysis. Sample: Paraffin-embedded mouse cervix. ETFA antibody dilution: 1:500.Application
ETFA antibody detects ETFA protein at mitochondria on mouse urinary bladder by immunohistochemical analysis. Sample: Paraffin-embedded mouse urinary bladder. ETFA antibody dilution: 1:500.Application
ETFA antibody detects ETFA protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with ETFA antibody diluted by 1:1000.Application
ETFA antibody detects ETFA protein at mitochondria on mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. ETFA antibody dilution: 1:500.Application
Immunoprecipitation of ETFA protein from HepG2 whole cell extracts using 5 μg of ETFA antibody . Western blot analysis was performed using ETFA antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.Application
Immunofluorescence analysis of methanol-fixed HeLa, using ETFA antibody at 1:200 dilution.Application
ETFA antibody detects ETFA protein by Western blot analysis. A. 30 µg C8D30 whole cell lysate/extract 10 % SDS-PAGE ETFA antibody dilution: 1:1000Application
ETFA antibody detects ETFA protein by Western blot analysis. A. 30 µg PC-12 whole cell lysate/extract 10 % SDS-PAGE ETFA antibody dilution: 1:1000| Product Name | ETFA antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ETFA (electron-transfer-flavoprotein, alpha polypeptide)ETFA participates in catalyzing the initial step of the mitochondrial fatty acid beta-oxidation. It shuttles electrons between primary flavoprotein dehydrogenases and the membrane-bound electron transfer flavoprotein ubiquinone oxidoreductase. Defects in electron-transfer-flavoprotein have been implicated in type II glutaricaciduria in which multiple acyl-CoA dehydrogenase deficiencies result in large excretion of glutaric, lactic, ethylmalonic, butyric, isobutyric, 2-methyl-butyric, and isovaleric acids. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ETFA. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Zebrafish, Xenopus laevis, Cat, Pig, Chicken, Rhesus Monkey, BovineZebrafish (84%), Xenopus laevis (85%), Cat (96%), Pig (96%), Chicken (87%), Rhesus Monkey (98%), Bovine (96%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | EMA antibody GA2 antibody MADD antibody ETFA antibody electron transfer flavoprotein alpha polypeptide antibody electron transfer flavoprotein alpha-subunit antibody electron transfer flavoprotein subunit alpha mitochondrial antibody alpha-ETF ant |
|---|---|
| Molecular Weight(MW) | 35 kDa (note) (3) |
| Cellular Localization | Mitochondrion matrix |
Application
ETFA antibody detects ETFA protein at mitochondria on mouse brain stem by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. ETFA antibody dilution: 1:500.
Application
ETFA antibody detects ETFA protein at mitochondria on mouse cervix by immunohistochemical analysis. Sample: Paraffin-embedded mouse cervix. ETFA antibody dilution: 1:500.
Application
ETFA antibody detects ETFA protein at mitochondria on mouse urinary bladder by immunohistochemical analysis. Sample: Paraffin-embedded mouse urinary bladder. ETFA antibody dilution: 1:500.
Application
ETFA antibody detects ETFA protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with ETFA antibody diluted by 1:1000.
Application
ETFA antibody detects ETFA protein at mitochondria on mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. ETFA antibody dilution: 1:500.
Application
Immunoprecipitation of ETFA protein from HepG2 whole cell extracts using 5 μg of ETFA antibody . Western blot analysis was performed using ETFA antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Application
Immunofluorescence analysis of methanol-fixed HeLa, using ETFA antibody at 1:200 dilution.
Application
ETFA antibody detects ETFA protein by Western blot analysis. A. 30 µg C8D30 whole cell lysate/extract 10 % SDS-PAGE ETFA antibody dilution: 1:1000
Application
ETFA antibody detects ETFA protein by Western blot analysis. A. 30 µg PC-12 whole cell lysate/extract 10 % SDS-PAGE ETFA antibody dilution: 1:1000| Positive Control | 293T , A431 , HeLa , HepG2 , C8D30 , PC-12 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
