Application
EZH2 Antibody flow cytometric analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of EZH2 Antibody in MDA-MB231(lane 1), Jurkat(lane 2), MDA-MB468(lane 3) cell line lysates (35ug/lane). EZH2 (arrow) was detected using the purified Pab.Application
Confocal immunofluorescent analysis of EZH2 Antibody with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).Application
Formalin-fixed and paraffin-embedded human testis tissue reacted with EZH2 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | EZH2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to EZH2This gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. This protein may play a role in the hematopoietic and central nervous systems. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq] |
| Immunogen | a recombinant protein from human EZH2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FACSICC/IFIHC-PWB |
| ELISA: Use at 1:1,000: |
|
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ENX-1 Histonelysine Nmethyltransferase EZH2 Lysine Nmethyltransferase6 Histone-lysine N-methyltransferase EZH2 Enhancer of zeste homolog 2 Lysine N-methyltransferase 6 Enhancer of zeste homolog2 EZH2 Lysine Nmethyltransferase 6 ENX1 KMT6 EZH2 E |
|---|---|
| Target | EZH2 |
| Molecular Weight(MW) | 85.363 kDa (note) |
Application
EZH2 Antibody flow cytometric analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of EZH2 Antibody in MDA-MB231(lane 1), Jurkat(lane 2), MDA-MB468(lane 3) cell line lysates (35ug/lane). EZH2 (arrow) was detected using the purified Pab.
Application
Confocal immunofluorescent analysis of EZH2 Antibody with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).
Application
Formalin-fixed and paraffin-embedded human testis tissue reacted with EZH2 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | ELISA: Use at 1:1,000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at at 2-8°C for up to 6 months. For long term storage, aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.14% (W/V) sodium azide |
