Application
Western blot analysis of extracts from 293 cells untreated or treated with EGF using Ezrin (Phospho-Tyr353) AntibodyApplication
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Ezrin (Phospho-Tyr353) Antibody (left) or the same antibody preincubated with blocking peptide (right).| Product Name | Ezrin (phospho Tyr353) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Ezrin (Phospho Tyr353)Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis. |
| Immunogen | Peptide sequence around phosphorylation site of tyrosine 353 (Q-D-Y(p)-E-E) derived from Human EZRIN. |
| Modification | p-Tyr353 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CVIL CVL DKFZp762H157 FLJ26216 MGC1584 VIL2 EZR villin-2 ezrin cytovillin 2 villin 2 (ezrin) p81 |
|---|---|
| Target | Ezrin (Phospho Tyr353) |
| Molecular Weight(MW) | 81 (note) |
Application
Western blot analysis of extracts from 293 cells untreated or treated with EGF using Ezrin (Phospho-Tyr353) Antibody
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Ezrin (Phospho-Tyr353) Antibody (left) or the same antibody preincubated with blocking peptide (right).| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
