Application
Sample (30 ug of whole cell lysate) A: Raji B: K562 7.5% SDS PAGE diluted at 1:3000Application
Factor B antibody [N2C2], Internal detects Factor B protein by western blot analysis. Rat tissue extracts (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Factor B antibody [N2C2], Internal diluted at 1:3000.Application
Immunohistochemical analysis of paraffin-embedded human hepatoma, using Factor B antibody at 1:500 dilution.| Product Name | Factor B antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Factor B (complement factor B)This gene encodes complement factor B, a component of the alternative pathway of complement activation. Factor B circulates in the blood as a single chain polypeptide. Upon activation of the alternative pathway, it is cleaved by complement factor D yielding the noncatalytic chain Ba and the catalytic subunit Bb. The active subunit Bb is a serine protease which associates with C3b to form the alternative pathway C3 convertase. Bb is involved in the proliferation of preactivated B lymphocytes, while Ba inhibits their proliferation. This gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. This cluster includes several genes involved in regulation of the immune reaction. Polymorphisms in this gene are associated with a reduced risk of age-related macular degeneration. The polyadenylation site of this gene is 421 bp from the 5' end of the gene for complement component 2. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Factor B. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanRat |
| Predicted Reactivity | Mouse, ChimpanzeeMouse (84%), Chimpanzee (98%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | AHUS4 antibody BF antibody BFD antibody CFAB antibody FB antibody FBI12 antibody GBG antibody H2-Bf antibody PBF2 antibody CFB antibody glycine-rich beta glycoprotein antibody properdin factor B antibody "B-factor properdin antibody" C3 proac |
|---|---|
| Molecular Weight(MW) | 86 kDa (note) |
| Cellular Localization | Secreted |
Application
Sample (30 ug of whole cell lysate) A: Raji B: K562 7.5% SDS PAGE diluted at 1:3000
Application
Factor B antibody [N2C2], Internal detects Factor B protein by western blot analysis. Rat tissue extracts (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Factor B antibody [N2C2], Internal diluted at 1:3000.
Application
Immunohistochemical analysis of paraffin-embedded human hepatoma, using Factor B antibody at 1:500 dilution.| Positive Control | Raji , K562 , Rat liver |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
