Application
Sample (30 µg of whole cell lysate) A:H1299 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunofluorescence analysis of paraformaldehyde-fixed A549, using Factor XIIIa antibody at 1:200 dilution.Application
Immunohistochemical analysis of paraffin-embedded H520 xenograft, using Factor XIIIa antibody at 1:500 dilution.Application
Factor XIIIa antibody [N1N3] detects Factor XIIIa protein at cytoplasm in mouse pancreas by immunohistochemical analysis. Sample: Paraffin-embedded mouse pancreas. Factor XIIIa antibody [N1N3] diluted at 1:500.Application
Factor XIIIa antibody [N1N3] detects Factor XIIIa protein at cytoplasm in mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. Factor XIIIa antibody [N1N3] diluted at 1:500.| Product Name | Factor XIIIa antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to Factor XIIIa (coagulation factor XIII, A1 polypeptide)This gene encodes the coagulation factor XIII A subunit. Coagulation factor XIII is the last zymogen to become activated in the blood coagulation cascade. Plasma factor XIII is a heterotetramer composed of 2 A subunits and 2 B subunits. The A subunits have catalytic function, and the B subunits do not have enzymatic activity and may serve as plasma carrier molecules. Platelet factor XIII is comprised only of 2 A subunits, which are identical to those of plasma origin. Upon cleavage of the activation peptide by thrombin and in the presence of calcium ion, the plasma factor XIII dissociates its B subunits and yields the same active enzyme, factor XIIIa, as platelet factor XIII. This enzyme acts as a transglutaminase to catalyze the formation of gamma-glutamyl-epsilon-lysine crosslinking between fibrin molecules, thus stabilizing the fibrin clot. It also crosslinks alpha-2-plasmin inhibitor, or fibronectin, to the alpha chains of fibrin. Factor XIII deficiency is classified into two categories: type I deficiency, characterized by the lack of both the A and B subunits; and type II deficiency, characterized by the lack of the A subunit alone. These defects can result in a lifelong bleeding tendency, defective wound healing, and habitual abortion. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human Factor XIIIa. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, BovineRat (84%), Bovine (86%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | F13A antibody F13A1 antibody protein-glutamine gamma-glutamyltransferase A chain antibody fibrinoligase antibody TGase antibody "FSF A subunit antibody" coagulation factor XIIIa antibody transglutaminase A chain antibody "fibrin stabilizing factor |
|---|---|
| Target | Factor XIIIa |
| Molecular Weight(MW) | 83 kDa (note) |
| Cellular Localization | Cytoplasm , Secreted |
Application
Sample (30 µg of whole cell lysate) A:H1299 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunofluorescence analysis of paraformaldehyde-fixed A549, using Factor XIIIa antibody at 1:200 dilution.
Application
Immunohistochemical analysis of paraffin-embedded H520 xenograft, using Factor XIIIa antibody at 1:500 dilution.
Application
Factor XIIIa antibody [N1N3] detects Factor XIIIa protein at cytoplasm in mouse pancreas by immunohistochemical analysis. Sample: Paraffin-embedded mouse pancreas. Factor XIIIa antibody [N1N3] diluted at 1:500.
Application
Factor XIIIa antibody [N1N3] detects Factor XIIIa protein at cytoplasm in mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. Factor XIIIa antibody [N1N3] diluted at 1:500.| Positive Control | H1299 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
