Application
Extracts of HeLa cells treated with 100 ng/mL Taxol for 24 hours were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF.| Product Name | FAK (phospho Ser722) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to FAK ( phospho S722 )Focal Adhesion Kinase is a non-receptor protein tyrosine kinase discovered as a substrate for Src and it is a key protein in integrin, growth factor and bioactive peptide signaling. Focal Adhesion Kinase plays a central role in cell spreading, differentiation, migration, cell death and acceleration of the G1 to S phase transition of the cell cycle. Focal Adhesion Kinase regulation includes phosphorylation at multiple tyrosine and serine residues. Phosphorylation of tyrosine generally is associated with positive regulation and growth promotion; however, dephosphorylation at these sites occurs as cells enter mitosis (M-Phase of the cell cycle). In contrast, serine phosphorylation either remains high or is increased as cells enter mitosis. The role of phosphorylation at Ser722 is currently being actively investigated. |
| Immunogen | Synthetic peptide phosphopeptide derived from the region of FAK that contains serine 722. |
| Modification | p-Ser722 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: Use at a concentration of 0.1 - 1.0 μg/ml. Predicted molecular weight: 125 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
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| Species Reactivity | HumanMouseRatXenopus |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | pp125FAK 5747 PTK2 FAK FAK1 Q05397 600758 FADK FRNK FAK (phospho S722) FAK (phospho Ser722) FAK (pS722) FAK (pSer722) FAK phospho S722 FAK phospho Ser722 Phospho FAK (S722) Phospho FAK (Ser722) Phospho FAK (pS722) Phospho FAK (pSer722) |
|---|---|
| Target | FAK |
| Tissue Specificity | Phosphorylation site-specific antibody (PSSA) selective for the phosphorylated form of the Focal Adhesion Kinase enzyme containing a phosphate on serine 722. The antibody has been shown to recognize FAK (approximately 125 kDa) in human epithelial carcinoma cells. |
| Cellular Localization | Cytoplasmic, localized to focal adhesions. |
Application
Extracts of HeLa cells treated with 100 ng/mL Taxol for 24 hours were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF.| Application Notes | WB: Use at a concentration of 0.1 - 1.0 μg/ml. Predicted molecular weight: 125 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
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| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.05% Sodium Azide; Constituents: PBS, 1.0mg/ml BSA (IgG, protease free). pH 7.4 |
