| Product Name | FAK (phospho Ser732) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to FAK ( phospho S732 )Focal Adhesion Kinase is a 125 kDa non-receptor protein tyrosine kinase that plays a key role in signalling by growth factors, extracellular matrix and stress signals. Indeed, Focal Adhesion Kinase plays a central role in cell spreading, differentiation, migration, cell death and acceleration of the G1 to S phase transition of the cell cycle. Cyclin-dependent kinase-5 (Cdk5), a serine/threonine kinase and an important regulator in brain development, has been found to be essential for phosphorylation at serine 732. In the context of the Focal Adhesion Kinase-Related Non-Kinase (FRNK), the c-terminal non-kinase domain of Focal Adhesion Kinase that acts as a dominant negative inhibitor of Focal Adhesion Kinase signalling, other serine kinases appear to readily phosphorylate this same site. The functional significance of phosphorylation on serine 732 is yet to be determined. |
| Immunogen | Synthetic peptide (Human) derived from the region of human Focal Adhesion Kinase that contains serine 732. The sequence is conserved in mouse, rat and chicken. |
| Modification | p-Ser732 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: Use at a concentration of 0.1 - 0.5 μg/ml. Predicted molecular weight: 125 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouseRatXenopus |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | pp125FAK 5747 PTK2 FAK FAK1 Q05397 600758 FADK FAK (phospho S732) FAK (phospho Ser732) FAK (pS732) FAK (pSer732) FAK phospho S732 FAK phospho Ser732 Phospho FAK (S732) Phospho FAK (Ser732) Phospho FAK (pS732) Phospho FAK (pSer732) |
|---|---|
| Target | FAK |
| Tissue Specificity | The antibody also reacts with FRNK. |
| Cellular Localization | Cytoplasmic, localized to focal adhesions. |
| Positive Control | Cdk5 +/- and cdk5 -/- mouse brain lysates |
|---|---|
| Application Notes | WB: Use at a concentration of 0.1 - 0.5 μg/ml. Predicted molecular weight: 125 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 °C |
| Storage Buffer | Preservative: 0.05% Sodium Azide; Constituents: 50% Glycerol, PBS, 1mg/ml BSA (IgG, protease free). pH 7.3 |
