Application
Extracts of human epithelial carcinoma cells expressing FAK with (2-5) or without (1) 100 ng/mL Taxol for 18-24 hours in serum-free media to arrest cells in mitosis were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF| Product Name | FAK (phospho Ser910) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to FAK ( phospho S910 )FAK is a non-receptor protein tyrosine kinase discovered as a substrate for Src and it is a key protein in integrin, growth factor and bioactive peptide signaling. FAK plays a central role in cell spreading, differentiation, migration, cell death and acceleration of the G1 to S phase transition of the cell cycle. FAK regulation includes phosphorylation at multiple tyrosine and serine residues. Phosphorylation of tyrosine generally is associated with positive regulation and growth promotion, however, dephosphorylation at these sites occurs as cells enter mitosis (M-Phase of the cell cycle). In contrast, serine phosphorylation either remains high or is increased as cells enter mitosis and may play a role in focal adhesion disassembly. |
| Immunogen | Synthetic peptide derived from the region of FAK that contains serine 910. The sequence is conserved in human, mouse, rat, chicken and frog. |
| Conjugation | Agarose |
| Modification | p-Ser910 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 |
|
| Species Reactivity | HumanMouseRatXenopus |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | pp125FAK 5747 PTK2 FAK FAK1 Q05397 600758 FADK FAK (phospho S910) FAK (phospho Ser910) FAK (pS910) FAK (pSer910) FAK phospho S910 FAK phospho Ser910 Phospho FAK (S910) Phospho FAK (Ser910) Phospho FAK (pS910) Phospho FAK (pSer910) |
|---|---|
| Target | FAK |
| Molecular Weight(MW) | 125 kDa |
| Tissue Specificity | Phosphorylation site-specific antibody (PSSA) selective for the phosphorylated form of the Focal Adhesion Kinase enzyme containing a phosphate on serine 910. The antibody has been shown to recognize FAK (approximately 125 kDa) in human epithelial carcinoma cells. |
| Cellular Localization | Cytoplasmic, localized to focal adhesions. |
Application
Extracts of human epithelial carcinoma cells expressing FAK with (2-5) or without (1) 100 ng/mL Taxol for 18-24 hours in serum-free media to arrest cells in mitosis were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF| Application Notes | WB:1:500~1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.05% Sodium Azide; Constituents: 50% Glycerol, PBS, 1mg/ml BSA (IgG, protease free). pH 7.3 |
