Application
Western blot analysis of Focal Adhesion Kinase (pY397) expression in HEK293T (A); MDA-MB-231 (B) whole cell lysates.Application
Immunohistochemical analysis of Focal Adhesion Kinase (pY397) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugad compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.| Product Name | FAK (phospho Tyr397) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to FAK (phospho Tyr397)This gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. Several transcript variants encoding different isoforms have been found for this gene, but the full-length natures of only three of them have been determined. [provided by RefSeq, Dec 2010] |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Focal Adhesion Kinase (pY397). The exact sequence is proprietary. |
| Modification | p-Tyr397 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| WB: 1:500 - 1:1000. IHC-P: 1:100 - 1:200: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | p125FAK FADK PTK2 FRNK FAK1 pp125FAK FAK PPP1R71 protein tyrosine kinase 2 |
|---|---|
| Target | FAK |
| Tissue Specificity | Recognizes endogenous levels of Focal Adhesion Kinase (pY397) protein. |
Application
Western blot analysis of Focal Adhesion Kinase (pY397) expression in HEK293T (A); MDA-MB-231 (B) whole cell lysates.
Application
Immunohistochemical analysis of Focal Adhesion Kinase (pY397) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugad compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.| Application Notes | WB: 1:500 - 1:1000. IHC-P: 1:100 - 1:200: |
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| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4 C. Upon delivery aliquot and store at -20 C for one year. Avoid freeze/thaw cycles. |
| Storage Buffer | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
