Application
WB ImageA. Western blot analysis on human brain tissue lysates (A B) and rat brain tissue lysates (C D) using anti-Phospho-Fak (pY397) RabMAb (cat. #) 1:2000 dilution. Cells were either (A C) untreated (B D) treated with AP| Product Name | FAK (phospho Tyr397) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit monoclonal antibody [EP2160Y] to FAK (phospho Tyr397)Focal adhesion kinase (FAK) is a non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis . FAK is composed of a central catalytic domain flanked by large N- and C-terminal regions. FAK is activated by phosphorylation at tyrosine 397 in response to integrin clustering which can be induced by cell adhesion or antibody cross-linking or via G-protein-coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid . Phosphorylation of FAK Tyr-397 creates a binding site for Src-family kinases, and phosphorylation of FAK Tyr-576/Tyr-577 in the kinase domain activation loop enhances catalytic activity . Increased FAK expression has been correlated with the enhanced motility and invasiveness of human tumor cells, as well as with promoting increased cell proliferation. |
| Immunogen | A phospho-specific peptide corresponding to residues surrounding Tyrosine 397 of human FAK was used as an immunogen. |
| Modification | p-Tyr397 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | EP2160Y |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| For WB: Use at a dilution of 1:1000-2000.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Gene Synonyms | pp125FAK FRNK PTK2 FAK FAK1 FADK FAK (phospho Y397) FAK (phospho Tyr397) FAK (pY397) FAK (pTyr397) FAK phospho Y397 FAK phospho Tyr397 Phospho FAK (Y397) Phospho FAK (Tyr397) Phospho FAK (pY397) Phospho FAK (pTyr397) |
|---|---|
| Target | FAK Phospho (pY397) |
| Molecular Weight(MW) | 119 kDa (note) |
| Tissue Specificity | This antibody detects FAK phosphorylated at Y397. |
Application
WB ImageA. Western blot analysis on human brain tissue lysates (A B) and rat brain tissue lysates (C D) using anti-Phospho-Fak (pY397) RabMAb (cat. #) 1:2000 dilution. Cells were either (A C) untreated (B D) treated with AP| Application Notes | For WB: Use at a dilution of 1:1000-2000.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 °C. Stable for 12 months from date of receipt. |
| Storage Buffer | PBS containing 50% Glycerol, 0.05% BSA and 0.01% Sodium azide. |
