| Product Name | FAK (phospho Tyr576) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to FAK ( phospho Y576 )Focal Adhesion Kinase is a 125 kDa non-receptor protein tyrosine kinase that was discovered as a substrate for Src, and is a key element of integrin signalling. Focal Adhesion Kinase plays a central role in cell spreading, differentiation, migration, cell death and acceleration of the G1 to S phase transition of the cell cycle. Tyrosines 576 and 577 are located in the kinase activation loop of Focal Adhesion Kinase and, when phosphorylated by Src, results in maximal activity. |
| Immunogen | Synthetic peptide (Human) derived from the region of human Focal Adhesion Kinase that contains tyrosine 576. The sequence is conserved in mouse, rat, chicken and frog. |
| Modification | p-Tyr576 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: Use at a concentration of 0.1 - 0.5 μg/ml. Predicted molecular weight: 125 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouseXenopus |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | pp125FAK 5747 PTK2 FAK FAK1 Q05397 600758 FADK FAK (phospho Y576) FAK (phospho Tyr576) FAK (pY576) FAK (pTyr576) FAK phospho Y576 FAK phospho Tyr576 Phospho FAK (Y576) Phospho FAK (Tyr576) Phospho FAK (pY576) Phospho FAK (pTyr576) |
|---|---|
| Target | FAK |
| Tissue Specificity | Some cross-reactivity with PYK 2 may still be experienced in cases where PYK 2 is overexpressed relative to Focal Adhesion Kinase within the same cell system. |
| Cellular Localization | Cytoplasmic, localized to focal adhesions. |
| Positive Control | Chicken embryo fibroblasts expressing Focal Adhesion Kinase protein and plated on fibronectin |
|---|---|
| Application Notes | WB: Use at a concentration of 0.1 - 0.5 μg/ml. Predicted molecular weight: 125 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Upon receipt - Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.05% Sodium Azide; Constituents: PBS, 1mg/ml BSA (IgG, protease free). pH 7.3 |
