| Product Name | FAK (phospho Tyr577) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to FAK ( phospho Y577 )Focal Adhesion Kinase is a 125 kDa non-receptor protein tyrosine kinase that acts as a substrate for Src and is a key element of integrin signalling. Focal Adhesion Kinase plays a central role in cell spreading, differentiation, migration, cell death and acceleration of the G1 to S phase transition of the cell cycle. The phosphorylation site pTyr577 is phosphorylated by Src. Tyrosine 576 and 577 are located in the catalytic position of Focal Adhesion Kinase and when phosphorylated, results in maximal activity. |
| Immunogen | Synthetic peptide derived from a region of Focal Adhesion Kinase that contains tyrosine 577. The sequence is conserved in human, mouse and frog. |
| Conjugation | Agarose |
| Modification | p-Tyr577 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | pp125FAK 5747 PTK2 FAK FAK1 Q05397 600758 FADK FAK (phospho Y577) FAK (phospho Tyr577) FAK (pY577) FAK (pTyr577) FAK phospho Y577 FAK phospho Tyr577 Phospho FAK (Y577) Phospho FAK (Tyr577) Phospho FAK (pY577) Phospho FAK (pTyr577) |
|---|---|
| Target | FAK |
| Molecular Weight(MW) | 125 kDa |
| Cellular Localization | Cytoplasmic, localized to focal adhesions. |
| Positive Control | Chicken embryo fibroblasts expressing Focal Adhesion Kinase protein and plated on fibronectin, or NIH 3T3 cells treated with PDGF |
|---|---|
| Application Notes | WB:1:500~1:2000 |
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.05% Sodium Azide; Constituents: PBS, 50% glycerol, 1mg/ml BSA (IgG, protease free). pH 7.4 |
