| Product Name | FAK (phospho Tyr861) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to FAK ( phospho Y861 )Focal Adhesion Kinase is a 125 kDa non-receptor protein tyrosine kinase that is a substrate for Src and a key element in growth factor and integrin signalling. Focal Adhesion Kinase plays a central role in cell spreading, differentiation, migration, cell death and acceleration of the G1 to S phase transition of the cell cycle. Tyr861 of Focal Adhesion Kinase is a major Src phosphorylation site that allows Focal Adhesion Kinase to bind to integrins and is also involved in cancer. |
| Immunogen | Synthetic peptide (Human) derived from the region of human Focal Adhesion Kinase that contains tyrosine 861. The sequence is conserved in human, mouse, rat, chicken and frog. |
| Modification | p-Tyr861 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-FWB |
| IHC-Fr: Use at an assay dependent dilution. WB: Use at a dilution of 1/1000. Predicted molecular weight: 125 kDa. Can be blocked with GTX25304 FAK peptide - phospho Y861 (phospho and non-phospho pair) or GTX25351 CEF whole cell lysate, positive / negative for Focal Adhesion Kinase (pair) . Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouseRatXenopus |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | pp125FAK 5747 PTK2 FAK FAK1 Q05397 600758 FADK FAK (phospho Y861) FAK (phospho Tyr861) FAK (pY861) FAK (pTyr861) FAK phospho Y861 FAK phospho Tyr861 Phospho FAK (Y861) Phospho FAK (Tyr861) Phospho FAK (pY861) Phospho FAK (pTyr861) |
|---|---|
| Target | FAK |
| Cellular Localization | Cytoplasmic, localized to focal adhesions. |
| Positive Control | Chicken embryo fibroblasts expressing Focal Adhesion Kinase protein and plated on fibronectin |
|---|---|
| Application Notes | IHC-Fr: Use at an assay dependent dilution. WB: Use at a dilution of 1/1000. Predicted molecular weight: 125 kDa. Can be blocked with GTX25304 FAK peptide - phospho Y861 (phospho and non-phospho pair) or GTX25351 CEF whole cell lysate, positive / negative for Focal Adhesion Kinase (pair) . Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.05% Sodium Azide; Constituents: 50% Glycerol, PBS, 1mg/ml BSA (IgG, protease free). pH 7.4 |
