Application
Immunofluorescence staining of methanol-fixed Hela cells using FAK(Phospho-Tyr925) AntibodyApplication
Western blot analysis of extracts from 293 cells using FAK(Phospho-Tyr925) Antibody (Lane 2) and the same antibody preincubated with blocking peptide(Lane1).| Product Name | FAK (phospho Tyr925) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to FAK (Phospho Tyr925)Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Plays a potential role in oncogenic transformations resulting in increased kinase activity. |
| Immunogen | Peptide sequence around phosphorylation site of tyrosine 925 (K-V-Y(p)-E-N) derived from Human FAK. |
| Modification | p-Tyr925 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | FADK FAK FAK1 FRNK pp125FAK PTK2 FADK 1 FAK-related non-kinase polypeptide protein phosphatase 1 regulatory subunit 71 focal adhesion kinase 1 PTK2 protein tyrosine kinase 2 |
|---|---|
| Target | FAK (Phospho Tyr925) |
| Molecular Weight(MW) | 125 (note) |
Application
Immunofluorescence staining of methanol-fixed Hela cells using FAK(Phospho-Tyr925) Antibody
Application
Western blot analysis of extracts from 293 cells using FAK(Phospho-Tyr925) Antibody (Lane 2) and the same antibody preincubated with blocking peptide(Lane1).| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
