Application
Western blot analysis of extracts from HT29, Hela and 293 cells using FAK(Ab-861) AntibodyApplication
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using FAK Antibody (left) or the same antibody preincubated with blocking peptide (right).Application
Western blot analysis of extracts from HT29, Hela and 293 cells using FAK Antibody .| Product Name | FAK antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to FAKNon-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Plays a potential role in oncogenic transformations resulting in increased kinase activity. |
| Immunogen | Peptide sequence around aa. 859~863 (H-I-Y-Q-P) derived from Human FAK. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: . Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | FADK FAK FAK1 FRNK pp125FAK PTK2 FADK 1 FAK-related non-kinase polypeptide protein phosphatase 1 regulatory subunit 71 focal adhesion kinase 1 PTK2 protein tyrosine kinase 2 |
|---|---|
| Target | FAK |
| Molecular Weight(MW) | 125 (note) |
Application
Western blot analysis of extracts from HT29, Hela and 293 cells using FAK(Ab-861) Antibody
Application
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using FAK Antibody (left) or the same antibody preincubated with blocking peptide (right).
Application
Western blot analysis of extracts from HT29, Hela and 293 cells using FAK Antibody .| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: . Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
