| Product Name | FAK antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to FAKFAK (Focal Adhesion Associated Protein-Tyrosine Kinase) is a widely expressed non receptor protein tyrosine kinase (PTK). FAK has a central catalytic domain and a C terminal tail that localizes it to focal adhesions, which are sites where cells attach to the extracellular matrix via surface integrin receptors. Increased FAK tyrosine phosphorylation occurs upon integrin engagement with fibronectin. Adhesion of murine NIH3T3 fibroblasts to fibronectin promotes association of the Grb 2 adapter protein and c Src PTK with FAK in vivo, and also results in activation of the ERK 2 MAP kinase. In v-Src-transformed NIH3T3, the association of v-Src, Grb 2, and Sos with FAK is independent of cell adhesion to fibronectin. In vitro the Grb 2 SH2 domain binds directly to tyrosine phosphorylated FAK, and the binding site has been identified as Tyr 925 by site directed mutagenesis. |
| Immunogen | Recombinant fragment, corresponding to amino acids 853-1052 of Human FAK. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| For WB: Use at a dilution of 1:1,000. Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Protein A |
| Gene Synonyms | pp125FAK 5747 PTK2 FAK FAK1 Q05397 600758 FADK |
|---|---|
| Target | FAK |
| Cellular Localization | Cytoplasmic, localized to focal adhesions. |
| Positive Control | HUVEC, SW156 or IMR-5 cells |
|---|---|
| Application Notes | For WB: Use at a dilution of 1:1,000. Optimal dilutions/concentrations should be determined by the researcher.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | 10mM Phosphate-buffered saline containing 0.2%BSA and 0.1% sodium azide |
