Application
Sample (30 µg of whole cell lysate) A: HepG2 B: Molt-4 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | FAP antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to FAP (fibroblast activation protein, alpha)The protein encoded by this gene is a homodimeric integral membrane gelatinase belonging to the serine protease family. It is selectively expressed in reactive stromal fibroblasts of epithelial cancers, granulation tissue of healing wounds, and malignant cells of bone and soft tissue sarcomas. This protein is thought to be involved in the control of fibroblast growth or epithelial-mesenchymal interactions during development, tissue repair, and epithelial carcinogenesis. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human FAP. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, BovineMouse (89%), Rat (89%), Bovine (93%) |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | DKFZp686G13158 antibody DPPIV antibody FAPA antibody FAP antibody 170 kDa melanoma membrane-bound gelatinase antibody integral membrane serine protease antibody seprase antibody "fibroblast activation protein alpha antibody" |
|---|---|
| Target | FAP |
| Molecular Weight(MW) | 88 kDa (note) (2) |
| Cellular Localization | Cell membrane; Single-pass type II membrane protein , Cell projection , lamellipodium membrane; Single-pass type II membrane protein , invadopodium membrane; Single-pass type II membrane protein |
Application
Sample (30 µg of whole cell lysate) A: HepG2 B: Molt-4 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | 293T , A431 , HepG2 , Molt-4 , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
