Application
Sample (30 ug of whole cell lysate) A: 293T 7.5% SDS PAGE diluted at 1:1000Application
FARSLA antibody [N2C2], Internal detects FARSLA protein at cytoplasm in mouse lung by immunohistochemical analysis. Sample: Paraffin-embedded mouse lung. FARSLA antibody [N2C2], Internal diluted at 1:500.Application
Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft , using FARSLA antibody at 1:500 dilution.Application
Immunofluorescence analysis of methanol-fixed HeLa, using FARSLA antibody at 1:200 dilution.| Product Name | FARSLA antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to FARSLA (phenylalanyl-tRNA synthetase, alpha subunit)Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. This gene encodes a product which is similar to the catalytic subunit of prokaryotic and Saccharomyces cerevisiae phenylalanyl-tRNA synthetases (PheRS). This gene product has been shown to be expressed in a tumor-selective and cell cycle stage- and differentiation-dependent manner, the first member of the tRNA synthetase gene family shown to exhibit this type of regulated expression [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human FARSLA. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Rhesus Monkey, Chimpanzee, BovineRat (93%), Rhesus Monkey (99%), Chimpanzee (100%), Bovine (94%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CML33 antibody FARSL antibody FARSLA antibody FRSA antibody PheHA antibody FARSA antibody "phenylalanine tRNA ligase 1 alpha cytoplasmic antibody" phenylalanine-tRNA synthetase alpha-subunit antibody pheRS antibody "phenylalanine-tRNA synthetase |
|---|---|
| Molecular Weight(MW) | 58 kDa (note) |
| Cellular Localization | Cytoplasm |
Application
Sample (30 ug of whole cell lysate) A: 293T 7.5% SDS PAGE diluted at 1:1000
Application
FARSLA antibody [N2C2], Internal detects FARSLA protein at cytoplasm in mouse lung by immunohistochemical analysis. Sample: Paraffin-embedded mouse lung. FARSLA antibody [N2C2], Internal diluted at 1:500.
Application
Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft , using FARSLA antibody at 1:500 dilution.
Application
Immunofluorescence analysis of methanol-fixed HeLa, using FARSLA antibody at 1:200 dilution.| Positive Control | 293T , H1299 , HeLa , HepG2 , Molt-4 , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
