Application
Application| Product Name | Fas antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to FasFAS / APO-1 (CD95) is an important member of the tumor necrosis factor (TNF) superfamily involved in membrane-mediated apoptosis. Ligation of Fas by FAS ligand or an anti-FAS cross-linking antibody, triggers activation of the caspase cascade (1). Functional impairment of the FAS / FAS-L system is associated with the development and progression of malignancies (2). FAS gene mutations have been suggested to have a role in testicular germ cell tumors (3). Tumor cells frequently exhibit de novo expression of FAS-ligand (FAS-L), which plays a significant role in local tissue destruction, metastatic spread, and immune escape of the tumor cells (8). The apoptosis of lymphocytes, which occurs in autoimmune diseases, is usually induced by the FAS/FAS-ligand system (7). FAS is believed to be involved in various autoimmune diseases including, ulcerative colitis, Graves disease, and rheumatoid arthritis (5,7). FAS expression on gastric epithelial cells in patients infected with H. Pylori is responsible for the accelerated apoptosis of the cells (4). Serum FAS-L concentration has also been shown to be associated with atherosclerosis and inflammatory disease, in patients with hypertension (6). |
| Immunogen | Synthetic peptide mapping near the carboxy terminal domain of human FAS. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-FIHC-PIPWB |
| For Western Blotting, use at a dilution of 1:200 Optimal dilutions should be determined by the user.: |
|
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Gene Synonyms | CD95 FAS1 APO1 Apo-1 Fas Apo1 Fas 355 APT1 FASTM P25445 134637 APO-1 ALPS1A FAS TNFRSF6 APO 1 Apo 1 Fas |
|---|---|
| Target | Fas |
| Cellular Localization | Type I membrane protein (isoform 1); Secreted (isoforms 2 to 6). |
Application
Application| Positive Control | Jurkat and A-431 lysate. |
|---|---|
| Application Notes | For Western Blotting, use at a dilution of 1:200 Optimal dilutions should be determined by the user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store this product at 4 °C, do not freeze. The product is stable for one year from the date of shipment. |
| Storage Buffer | Preservative: 0.1% Sodium Azide; Constituents: 0.2% Gelatin, PBS |
