Application
Immunohistochemical staining of human hepatoma tissue with Fas pAb.Application
Western blot analysis of Fas pAb : Lane 1: Rat Brain Tissue Extract Lane 2: Mouse Brain Tissue Extract| Product Name | Fas antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to FasCD95, also known as FAS or APO-1, is a 36 kDa cell surface type I- membrane glycoprotein with an apparent molecular weight of 44 kDa on SDS-PAGE. CD95 is a member of the TNF receptor family, which includes TNFR-1, TNFR-2, CD27, CD30 and CD40. Binding of CD95 Ligand to CD95 or crosslinking of CD95 by anti-CD95 monoclonal antibodies leads to apoptosis of CD95 expressing cells. CD95 belongs to a subgroup of family members that have a death domain (DD) which contains 70 amino acids near the carboxyl-terminal region of the molecule. The binding of adaptor molecules to this DD is responsible for transmitting the death signal for apoptosis. Stimulation of CD95 results in aggregation of its DD, leading to the recruitment of FADD and caspase-8 that together with the receptor form the death-inducing signaling complex (DISC). |
| Immunogen | Synthetic peptide: ESLKLRRRVHETDKNC conjugated to KLH, corresponding to amino acids 29-44 of Mouse CD95 . |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FACSIHCWB |
| FACS: Use at an assay dependent dilution.: |
|
| Species Reactivity | HumanMouseDogRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CD95 FAS1 AI196731 APO1 Fas TNFR6 Apo-1 Fas Apo1 Fas 14102 Tnfrsf6 355 APT1 FASTM P25445 134637 lpr APO-1 ALPS1A APO 1 Apo 1 Fas |
|---|---|
| Target | Fas |
| Cellular Localization | Type I membrane protein (isoform 1); Secreted (isoforms 2 to 6). |
Application
Immunohistochemical staining of human hepatoma tissue with Fas pAb.
Application
Western blot analysis of Fas pAb : Lane 1: Rat Brain Tissue Extract Lane 2: Mouse Brain Tissue Extract| Positive Control | HeLa Cell Lysate. |
|---|---|
| Application Notes | FACS: Use at an assay dependent dilution.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.09% Sodium Azide; Constituents: PBS. pH 7.2 |
