Application
Western blot analysis of extracts from HepG2, Hela and 293 cells using Fas Antibody .| Product Name | Fas antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to FasReceptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro). |
| Immunogen | Peptide sequence around aa.323~327 (E-N-S-N-F) derived from human Fas . |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| Suggested starting dilutions are as follows. WB: 1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | ALPS1A APO-1 APT1 CD95 FAS1 FASTM TNFRSF6 FAS tumor necrosis factor receptor superfamily member 6 FAS 827dupA apoptosis-mediating surface antigen FAS Fas AMA FASLG receptor APO-1 cell surface antigen CD95 antigen tumor necrosis factor recept |
|---|---|
| Target | Fas |
| Molecular Weight(MW) | 40-50 (note) |
Application
Western blot analysis of extracts from HepG2, Hela and 293 cells using Fas Antibody .| Application Notes | Suggested starting dilutions are as follows. WB: 1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
