Application
Wild-type (WT) and Fascin 1 knownout (KO) HeLa cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Fascin 1 antibody [N2C2], Internal diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.Application
Sample (30 ug of whole cell lysate) A: HeLa B: Hep G2 7.5% SDS PAGE diluted at 1:1000| Product Name | Fascin 1 antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to Fascin (fascin homolog 1, actin-bundling protein (Strongylocentrotus purpuratus))Organizes filamentous actin into bundles with a minimum of 4.1:1 actin/fascin ratio. Probably involved in the assembly of actin filament bundles present in microspikes, membrane ruffles, and stress fibers. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Fascin 1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Pig, Chicken, BovineMouse (97%), Rat (96%), Pig (96%), Chicken (84%), Bovine (95%) |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | FLJ38511 antibody SNL antibody p55 antibody FSCN1 antibody "singed-like (fascin homolog sea urchin) antibody" fascin antibody 55 kDa actin-bundling protein antibody "fascin homolog 1 actin-bundling protein (Strongylocentrotus purpuratus) antibody" |
|---|---|
| Target | Fascin |
| Molecular Weight(MW) | 55 kDa (note) |
Application
Wild-type (WT) and Fascin 1 knownout (KO) HeLa cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Fascin 1 antibody [N2C2], Internal diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Application
Sample (30 ug of whole cell lysate) A: HeLa B: Hep G2 7.5% SDS PAGE diluted at 1:1000| Positive Control | HeLa , HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
