Application
Sample (1ug of whole cell lysate) A: Rat muscle 15% SDS PAGE diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Sample (whole cell lysate) A: 1ug mouse heart B: 5ug mouse heart 15% SDS PAGE diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Sample (30 µg of whole cell lysate) A: HeLa 15% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Fast skeletal myosin light chain 1 antibody at 1:500 dilution.| Product Name | Fast skeletal myosin light chain 1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Fast skeletal myosin light chain 1 (myosin, light chain 1, alkali; skeletal, fast)Myosin is a hexameric ATPase cellular motor protein. It is composed of two heavy chains, two nonphosphorylatable alkali light chains, and two phosphorylatable regulatory light chains. This gene encodes a myosin alkali light chain expressed in fast skeletal muscle. Two transcript variants have been identified for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Fast skeletal myosin light chain 1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Xenopus laevis, Pig, Rabbit, Chicken, BovineXenopus laevis (82%), Pig (94%), Rabbit (92%), Chicken (82%), Bovine (91%) |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | MLC1F antibody MLC3F antibody MYL1 antibody MLC1F/MLC3F antibody myosin light chain alkali 1/2 antibody "myosin light polypeptide 1 alkali skeletal fast antibody" A2 catalytic antibody MLC1/MLC3 antibody myosin light chain A1/A2 antibody "myo |
|---|---|
| Molecular Weight(MW) | 21 kDa (note) |
Application
Sample (1ug of whole cell lysate) A: Rat muscle 15% SDS PAGE diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Sample (whole cell lysate) A: 1ug mouse heart B: 5ug mouse heart 15% SDS PAGE diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Sample (30 µg of whole cell lysate) A: HeLa 15% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Fast skeletal myosin light chain 1 antibody at 1:500 dilution.| Positive Control | HeLa , mouse heart , rat muscle |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
