Rabbit polyclonal antibody to Fbx32This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class and contains an F-box domain. This protein is highly expressed during muscle atrophy, whereas mice deficient in this gene were found to be resistant to atrophy. This protein is thus a potential drug target for the treatment of muscle atrophy. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jun 2011]
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
ELISAIHC-PWB
P-ELISA:1:500-1000, WB:1:100-500, IHC-P:1:100-500 (based on 0.5 mg/ml)
Species Reactivity
HumanMouseDogPigRatHorseBovine
Concentration
0.5mg/ml
Purification
Affinity purified
Target Information
Gene Synonyms
FBXO32 F-box protein 32 MAFbx
Application
Application
IHC-P analysis of rat uterus tissue using Fbx32 antibody at a dilution of 1:200.
Application Notes
P-ELISA:1:500-1000, WB:1:100-500, IHC-P:1:100-500 (based on 0.5 mg/ml)
Additional Information
Form
Liquid
Storage Instructions
Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles.
Storage Buffer
10 mM PBS, pH 7.4 with 10 mg/ml BSA and 0.1% sodium azide.