Application
WB analysis of 293T cells using FBXO11 antibody at 0.2-1μg/ml.Application
WB analysis of human ovary tumor tissue using FBXO11 antibody at 1μg/ml.Application
ChIP analysis of HCT116 cells using FBXO11 antibody. Use 0.5μg antibody FBXO11 antibody for ChIP assay. Quiescent human colon carcinoma HCT116 cultures were treated with 10% FBS for three time points (0, 15, 30min) or (0, 30, 60min) were used in Matrix-ChIP and real-time PCR assays at EGR1 gene (Exon1) and 15kb upstream site. (+) : Add blocking peptide (-) : no blocking peptide| Product Name | FBXO11 antibody, Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to FBXO11This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. It can function as an arginine methyltransferase that symmetrically dimethylates arginine residues, and it acts as an adaptor protein to mediate the neddylation of p53, which leads to the suppression of p53 function. This gene is known to be down-regulated in melanocytes from patients with vitiligo, a skin disorder that results in depigmentation. Polymorphisms in this gene are associated with chronic otitis |
| Immunogen | A synthetic peptide corresponding to an Internal region of Human FBXO11 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBChIP |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 100% |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | FBX11 PRMT9 FBXO11 VIT1 UG063H01 UBR6 |
|---|---|
| Target | FBXO11 |
| Molecular Weight(MW) | 104 KDa (note) |
Application
WB analysis of 293T cells using FBXO11 antibody at 0.2-1μg/ml.
Application
WB analysis of human ovary tumor tissue using FBXO11 antibody at 1μg/ml.
Application
ChIP analysis of HCT116 cells using FBXO11 antibody. Use 0.5μg antibody FBXO11 antibody for ChIP assay. Quiescent human colon carcinoma HCT116 cultures were treated with 10% FBS for three time points (0, 15, 30min) or (0, 30, 60min) were used in Matrix-ChIP and real-time PCR assays at EGR1 gene (Exon1) and 15kb upstream site. (+) : Add blocking peptide (-) : no blocking peptide| Positive Control | 293T , human ovary tumor |
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| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. For short-term storage, store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
