Application
Sample (30 µg of whole cell lysate) A: A549 10% SDS PAGE diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunofluorescence analysis of methanol-fixed HeLa, using FDPS antibody at 1:200 dilution.Application
Sample (50 µg of whole cell lysate) A: mouse brain 10% SDS PAGE diluted at 1:3000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
FDPS antibody [N1C3] detects FDPS protein at cytosol on mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. FDPS antibody [N1C3] dilution: 1:500.Application
FDPS antibody detects FDPS protein by western blot analysis. A. 50 µg rat liver lysate/extract 10% SDS-PAGE FDPS antibody dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | FDPS antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to FDPS (farnesyl diphosphate synthase (farnesyl pyrophosphate synthetase, dimethylallyltranstransferase, geranyltranstransferase))This gene encodes an enzyme that catalyzes the production of geranyl pyrophosphate and farnesyl pyrophosphate from isopentenyl pyrophosphate and dimethylallyl pyrophosphate. The resulting product, farnesyl pyrophosphate, is a key intermediate in cholesterol and sterol biosynthesis, a substrate for protein farnesylation and geranylgeranylation, and a ligand or agonist for certain hormone receptors and growth receptors. Drugs that inhibit this enzyme prevent the post-translational modifications of small GTPases and have been used to treat diseases related to bone resorption. Multiple pseudogenes have been found on chromosomes 1, 7, 14, 15, 21 and X. Multiple transcript variants encoding different isoforms have been found for this gene. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human FDPS. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Pig, BovineRat (89%), Pig (89%), Bovine (91%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | FPPS antibody, FPS antibody, FDPS antibody, farnesyl pyrophosphate synthase antibody, geranyltranstransferase antibody, "(2E,6E)-farnesyl diphosphate synthase antibody", FPP synthase antibody, dimethylallyltranstransferase antibody, FPP synthetase antibody |
|---|---|
| Molecular Weight(MW) | 48 kDa (note) (4) |
| Cellular Localization | Cytoplasm |
Application
Sample (30 µg of whole cell lysate) A: A549 10% SDS PAGE diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunofluorescence analysis of methanol-fixed HeLa, using FDPS antibody at 1:200 dilution.
Application
Sample (50 µg of whole cell lysate) A: mouse brain 10% SDS PAGE diluted at 1:3000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
FDPS antibody [N1C3] detects FDPS protein at cytosol on mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. FDPS antibody [N1C3] dilution: 1:500.
Application
FDPS antibody detects FDPS protein by western blot analysis. A. 50 µg rat liver lysate/extract 10% SDS-PAGE FDPS antibody dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | A549 , HeLa , HepG2 , HCT116 , mouse brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
