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Immunohistochemistry (IHC) 1:50-1:100 using polymer or streptavidin-biotin system. For formalin-fixed paraffin-embedded (FFPE) tissue sections and cell smears better staining is observed when antigen retrieval is used (boiling the tissue in 10 mM citrate, pH 6.0 for 10-15 minutes, followed by cooling for 10-15 minutes). |