| Product Name | Ferritin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to FerritinThis polyclonal reacts with ferritin isolated from human spleen. Ferritin is a main iron storage protein. The antibody may facilitate the analysis of ferritin in serum for an iron nutritional determination. Low values of iron are associated with iron deficiency, while high levels may indicate a malignant disease, tissue damage and other iron overload. |
| Immunogen | Ferritin [Human Spleen] |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | DotELISAIPWB |
| For Dot: Use at a dilution of 1:2,000-1:5,000. For ELISA: Use at a dilution of 1:5,000-1:20,000. For IP: Use at an assay dependent dilution. For WB: Use at a dilution of 1:2,000-1:5,000. Expected to work in Immunohistochemistry, not yet tested in house. Optimal dilutions/concentrations should be determined by the end user. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Gene Synonyms | 2512, FTMT, Ferritin, 94033, 134790, MGC71996, FTL, P02792, 608847, Q8N4E7, MTF |
|---|---|
| Target | Ferritin |
| Tissue Specificity | Ferritin has two subunits, heart and liver, that are 21 and 19 kD respectively. There are 25 different isoforms of ferritin combining different combinations of the two. Spleen is one of these combinations. The native molecule is 420-450 kDa, depending on the isoform. On a very long SDS-PAGE gel it is possible to resolve the two subunits; otherwise only a band of ~20 kD is seen. There might also be a band around 25 kD which is a charge variant. Moreover, a dimer at 40 kD can sometimes be seen. |
| Cellular Localization | Cytoplasmic and Mitochondrial |
| Application Notes | For Dot: Use at a dilution of 1:2,000-1:5,000. For ELISA: Use at a dilution of 1:5,000-1:20,000. For IP: Use at an assay dependent dilution. For WB: Use at a dilution of 1:2,000-1:5,000. Expected to work in Immunohistochemistry, not yet tested in house. Optimal dilutions/concentrations should be determined by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles. |
| Storage Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 0.01% sodium azide |
