Application
Immunohistochemical analysis of paraffin-embedded Ca922 xenograft, using Ferritin Light Chain antibody at 1:100 dilution.Application
Mouse tissue extract (50 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with Ferritin Light Chain antibody [N1C3-3] diluted at 1:1000.Application
Sample (30 ug of whole cell lysate) A: Hep G2 12% SDS PAGE diluted at 1:1000| Product Name | Ferritin Light Chain antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Ferritin Light Chain (ferritin, light polypeptide)This gene encodes the light subunit of the ferritin protein. Ferritin is the major intracellular iron storage protein in prokaryotes and eukaryotes. It is composed of 24 subunits of the heavy and light ferritin chains. Variation in ferritin subunit composition may affect the rates of iron uptake and release in different tissues. A major function of ferritin is the storage of iron in a soluble and nontoxic state. Defects in this light chain ferritin gene are associated with several neurodegenerative diseases and hyperferritinemia-cataract syndrome. This gene has multiple pseudogenes. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Ferritin Light Chain. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Dog, Cat, Pig, Rabbit, Rhesus Monkey, Bovine, Guinea pigDog (87%), Cat (86%), Pig (88%), Rabbit (86%), Rhesus Monkey (97%), Bovine (84%), Guinea pig (84%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | MGC71996 antibody, NBIA3 antibody, FTL antibody, ferritin light chain antibody, ferritin L subunit antibody, ferritin light polypeptide-like 3 antibody, ferritin L-chain antibody, "ferritin, light polypeptide antibody" |
|---|---|
| Molecular Weight(MW) | 20 kDa (note) |
Application
Immunohistochemical analysis of paraffin-embedded Ca922 xenograft, using Ferritin Light Chain antibody at 1:100 dilution.
Application
Mouse tissue extract (50 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with Ferritin Light Chain antibody [N1C3-3] diluted at 1:1000.
Application
Sample (30 ug of whole cell lysate) A: Hep G2 12% SDS PAGE diluted at 1:1000| Positive Control | HepG2 , mouse liver |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
