Application
Sample (30 ug of whole cell lysate) A: U87-MG 7.5% SDS PAGE diluted at 1:1000Application
Immunohistochemical analysis of paraffin-embedded human breast cancer, using FEZ1 antibody at 1:250 dilution.| Product Name | FEZ1 antibody [C1C3] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to FEZ1 (leucine zipper, putative tumor suppressor 1)This gene encodes a tumor suppressor protein that is ubiquitously expressed in normal tissues. In uveal melanomas, expression of this protein is silenced in rapidly metastasizing and metastatic tumor cells but has normal expression in slowly metastasizing or nonmetastasizing tumor cells. This protein may have a role in cell-cycle control by interacting with the Cdk1/cyclinB1 complex. This gene is located on chromosomal region 8p22. Loss of heterozygosity (LOH) in the 8p arm is a common characteristic of many types of cancer. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human FEZ1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, BovineMouse (89%), Rat (92%), Bovine (90%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | F37 antibody, FEZ1 antibody, LZTS1 antibody, F37/Esophageal cancer-related gene-coding leucine-zipper motif antibody, leucine zipper putative tumor suppressor 1 antibody, "leucine zipper, putative tumor suppressor 1 antibody" |
|---|---|
| Molecular Weight(MW) | 67 kDa (note) (2) |
| Cellular Localization | Cytoplasm (By similarity) , Cell membrane (By similarity) , Cell projection , dendritic spine (By similarity) , Cell junction , synapse , postsynaptic cell membrane , postsynaptic density (By similarity) , synapse (By similarity) |
Application
Sample (30 ug of whole cell lysate) A: U87-MG 7.5% SDS PAGE diluted at 1:1000
Application
Immunohistochemical analysis of paraffin-embedded human breast cancer, using FEZ1 antibody at 1:250 dilution.| Positive Control | U87-MG |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
