Application
Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FGD1 antibody diluted at 1:500.| Product Name | FGD1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to FGD1 (FYVE, RhoGEF and PH domain containing 1)FGD1 contains Dbl (DH) and pleckstrin (PH) homology domains. It can bind specifically to the Rho family GTPase Cdc42Hs and stimulate the GDP-GTP exchange of the isoprenylated form of Cdc42Hs. It also stimulates the mitogen activated protein kinase cascade leading to c-Jun kinase SAPK/JNK1 activation. FGD1 has an essential role in embryonic development, and FGD1 gene mutations result in the human developmental disorder, Aarskog-Scott syndrome. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human FGD1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, BovineMouse (98%), Rat (98%), Bovine (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | AAS antibody, FGDY antibody, MRXS16 antibody, ZFYVE3 antibody, FGD1 antibody, zinc finger FYVE domain-containing protein 3 antibody, rho/Rac GEF antibody, "FYVE, RhoGEF and PH domain-containing protein 1 antibody", rho/Rac guanine nucleotide exchange facto |
|---|---|
| Target | FGD1 |
| Molecular Weight(MW) | 107 kDa (note) |
| Cellular Localization | Cytoplasm (By similarity) , Cell projection , lamellipodium (By similarity) , ruffle (By similarity) , Cytoplasm , cytoskeleton (By similarity) |
Application
Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FGD1 antibody diluted at 1:500.| Positive Control | Target recombinant protein , U87-MG , SK-N-SH , IMR32 , SK-N-AS |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
