Application
Sample (30 ug of whole cell lysate) A: Molt-4 7.5% SDS PAGE diluted at 1:1000Application
FGD4 antibody [N1N3] detects FGD4 protein at cytoplasm in human lung cancer by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. FGD4 antibody [N1N3] diluted at 1:500.Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using FGD4 antibody at 1:200 dilution.Application
FGD4 antibody [N1N3] detects FGD4 protein at cytoplasm in human endometrial cancer by immunohistochemical analysis. Sample: Paraffin-embedded human endometrial cancer. FGD4 antibody [N1N3] diluted at 1:500.| Product Name | FGD4 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to FGD4 (FYVE, RhoGEF and PH domain containing 4)This gene encodes a protein that is involved in the regulation of the actin cytoskeleton and cell shape. This protein contains an actin filament-binding domain, which together with its Dbl homology domain and one of its pleckstrin homology domains, can form microspikes. This protein can activate MAPK8 independently of the actin filament-binding domain, and it is also involved in the activation of CDC42 via the exchange of bound GDP for free GTP. The activation of CDC42 also enables this protein to play a role in mediating the cellular invasion of Cryptosporidium parvum, an intracellular parasite that infects the gastrointestinal tract. Mutations in this gene can cause Charcot-Marie-Tooth disease type 4H (CMT4H), a disorder of the peripheral nervous system. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human FGD4. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CMT4H antibody, DKFZp313E1818 antibody, DKFZp434K1572 antibody, FLJ34370 antibody, FLJ42663 antibody, FRABP antibody, MGC57222 antibody, ZFYVE6 antibody, FGD4 antibody, "FYVE, RhoGEF and PH domain-containing protein 4 antibody", FGD1-related F-actin-bindin |
|---|---|
| Target | FGD4 |
| Molecular Weight(MW) | 87 kDa (note) (5) |
| Cellular Localization | Cytoplasm , cytoskeleton (By similarity) , Cell projection , filopodium (By similarity) |
Application
Sample (30 ug of whole cell lysate) A: Molt-4 7.5% SDS PAGE diluted at 1:1000
Application
FGD4 antibody [N1N3] detects FGD4 protein at cytoplasm in human lung cancer by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. FGD4 antibody [N1N3] diluted at 1:500.
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using FGD4 antibody at 1:200 dilution.
Application
FGD4 antibody [N1N3] detects FGD4 protein at cytoplasm in human endometrial cancer by immunohistochemical analysis. Sample: Paraffin-embedded human endometrial cancer. FGD4 antibody [N1N3] diluted at 1:500.| Positive Control | 293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
