Application
Fibrinogen beta antibody [C1C3] detects Fibrinogen beta protein at cytoplasm in rat liver by immunohistochemical analysis. Sample: Paraffin-embedded rat liver. Fibrinogen beta antibody [C1C3] diluted at 1:500.Application
Mouse tissue extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Fibrinogen beta antibody [C1C3] diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Rat tissue extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Fibrinogen beta antibody [C1C3] diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Human tissue extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Fibrinogen beta antibody [C1C3] diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunofluorescence analysis of methanol-fixed HeLa, using Fibrinogen beta antibody at 1:200 dilution.| Product Name | Fibrinogen beta antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Fibrinogen beta (fibrinogen beta chain)The protein encoded by this gene is the beta component of fibrinogen, a blood-borne glycoprotein comprised of three pairs of nonidentical polypeptide chains. Following vascular injury, fibrinogen is cleaved by thrombin to form fibrin which is the most abundant component of blood clots. In addition, various cleavage products of fibrinogen and fibrin regulate cell adhesion and spreading, display vasoconstrictor and chemotactic activities, and are mitogens for several cell types. Mutations in this gene lead to several disorders, including afibrinogenemia, dysfibrinogenemia, hypodysfibrinogenemia and thrombotic tendency. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Fibrinogen beta. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Xenopus laevis, Pig, Chicken, BovineXenopus laevis (81%), Pig (88%), Chicken (82%), Bovine (87%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | MGC104327 antibody MGC120405 antibody FGB antibody fibrinogen beta chain antibody "fibrinogen B beta polypeptide antibody" |
|---|---|
| Molecular Weight(MW) | 56 kDa (note) (3) |
| Cellular Localization | Secreted |
Application
Fibrinogen beta antibody [C1C3] detects Fibrinogen beta protein at cytoplasm in rat liver by immunohistochemical analysis. Sample: Paraffin-embedded rat liver. Fibrinogen beta antibody [C1C3] diluted at 1:500.
Application
Mouse tissue extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Fibrinogen beta antibody [C1C3] diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Rat tissue extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Fibrinogen beta antibody [C1C3] diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Human tissue extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Fibrinogen beta antibody [C1C3] diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunofluorescence analysis of methanol-fixed HeLa, using Fibrinogen beta antibody at 1:200 dilution.| Positive Control | human plasma , mouse plasma , rat plasma , *human platelet |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
