| Product Name | Fibronectin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to FibronectinThis gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants. However, the full-length nature of some variants has not been determined. [provided by RefSeq, Jul 2008] |
| Immunogen | Purified human Fibronectin from human plasma. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC |
| Immunohistochemistry (IHC) 1:100-1:200 for 30 minute at room temperature using polymer system or streptavidin-biotin system. Formalin-fixed paraffin-embedded (FFPE) tissue sections and cell smears require antigen retriever, boiling tissue in 10 mM citrate buffer, pH 6.0 for 10-20 minute, followed by cooling at room temperature for 20 minutes.: |
|
| Species Reactivity | HumanPig |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Synonyms | GFND MSF GFND2 CIG FN fibronectin 1 LETS FINC ED-B FN1 FNZ |
|---|---|
| Target | Fibronectin |
| Cellular Localization | Connective tissue matrix and basement membrane. |
| Positive Control | Human kidney |
|---|---|
| Application Notes | Immunohistochemistry (IHC) 1:100-1:200 for 30 minute at room temperature using polymer system or streptavidin-biotin system. Formalin-fixed paraffin-embedded (FFPE) tissue sections and cell smears require antigen retriever, boiling tissue in 10 mM citrate buffer, pH 6.0 for 10-20 minute, followed by cooling at room temperature for 20 minutes.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 2-8°C. Do not freeze. |
| Storage Buffer | 10 mM PO4, 150 mM NaCl, pH 7.4, containing 1% BSA and 0.05% sodium azide. |
