Application
FIP200 antibody detects FIP200 protein by western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg whole cell lysate/extract of human FIP200-transfected 293T cells 5 % SDS-PAGE FIP200 antibody dilution: 1:5000Application
FIP200 antibody detects FIP200 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 10 min. Green: FIP200 protein stained by FIP200 antibody diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 μm.Application
FIP200 antibody detects FIP200 protein by western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg A431 whole cell lysate/extract 5 % SDS-PAGE FIP200 antibody dilution: 1:1000| Product Name | FIP200 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to RB1CC1 (RB1-inducible coiled-coil 1)The protein encoded by this gene interacts with signaling pathways to coordinately regulate cell growth, cell proliferation, apoptosis, autophagy, and cell migration. This tumor suppressor also enhances retinoblastoma 1 gene expression in cancer cells. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human FIP200. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Rhesus Monkey, BovineMouse (86%), Rat (87%), Rhesus Monkey (98%), Bovine (83%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | FIP200 RB1-inducible coiled-coil 1 CC1 ATG17 antibody |
|---|---|
| Target | RB1CC1 |
| Molecular Weight(MW) | 183 kDa (note) (2) |
| Cellular Localization | Nucleus |
Application
FIP200 antibody detects FIP200 protein by western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg whole cell lysate/extract of human FIP200-transfected 293T cells 5 % SDS-PAGE FIP200 antibody dilution: 1:5000
Application
FIP200 antibody detects FIP200 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 10 min. Green: FIP200 protein stained by FIP200 antibody diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
Application
FIP200 antibody detects FIP200 protein by western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg A431 whole cell lysate/extract 5 % SDS-PAGE FIP200 antibody dilution: 1:1000| Positive Control | 293T , A431 , human FIP200-transfected 293T |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
