Application
Immunohistochemical analysis of paraffin-embedded BT474 xenograft, using Flotillin 2 antibody at 1:100 dilution.Application
Flotillin 2 antibody [N1C1] immunoprecipitates Flotillin 2 protein in IP experiments. IP samples: HCT-116 whole cell extract A. Control with 4 μg of preimmune Rabbit IgG B. Immunoprecipitation of Flotillin 2 protein by 4 μg Flotillin 2 antibody [N1C1] 10 % SDS-PAGE The immunoprecipitated Flotillin 2 protein was detected by Flotillin 2 antibody [N1C1] diluted at 1:1000. [EasyBlot anti-rabbit IgG was used as a secondary reagent]Application
Sample (50 ug of whole cell lysate) A: mouse brain 10% SDS PAGE diluted at 1:1000Application
Sample (30 ug of whole cell lysate) A: HCT116 10% SDS PAGE Flotillin-2 antibody diluted at 1:1000| Product Name | Flotillin 2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Flotillin 2 (flotillin 2)Caveolae are small domains on the inner cell membrane involved in vesicular trafficking and signal transduction. This gene encodes a caveolae-associated, integral membrane protein, which is thought to function in neuronal signaling. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Flotillin 2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Xenopus laevis, BovineRat (100%), Xenopus laevis (91%), Bovine (99%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ECS-1 antibody ECS1 antibody ESA antibody ESA1 antibody M17S1 antibody FLOT2 antibody membrane component chromosome 17 surface marker 1 antibody flotillin-2 antibody epidermal surface antigen antibody "membrane component chromosome 17 surface ma |
|---|---|
| Molecular Weight(MW) | 47 kDa (note) (3) |
| Cellular Localization | Cell membrane; Peripheral membrane protein , Membrane , caveola; Peripheral membrane protein |
Application
Immunohistochemical analysis of paraffin-embedded BT474 xenograft, using Flotillin 2 antibody at 1:100 dilution.
Application
Flotillin 2 antibody [N1C1] immunoprecipitates Flotillin 2 protein in IP experiments. IP samples: HCT-116 whole cell extract A. Control with 4 μg of preimmune Rabbit IgG B. Immunoprecipitation of Flotillin 2 protein by 4 μg Flotillin 2 antibody [N1C1] 10 % SDS-PAGE The immunoprecipitated Flotillin 2 protein was detected by Flotillin 2 antibody [N1C1] diluted at 1:1000. [EasyBlot anti-rabbit IgG was used as a secondary reagent]
Application
Sample (50 ug of whole cell lysate) A: mouse brain 10% SDS PAGE diluted at 1:1000
Application
Sample (30 ug of whole cell lysate) A: HCT116 10% SDS PAGE Flotillin-2 antibody diluted at 1:1000| Positive Control | A549 , HeLa , HepG2 , HCT116 , mouse brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
