Application
Western Blot analysis of FPGS expression in transfected 293T cell line by FPGS polyclonal antibody. A: Non-transfected lysate. B: FPGS transfected lysate. 7.5% SDS PAGE diluted at 1:1000| Product Name | Folylpolyglutamate synthase antibody , Internal |
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| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to Folylpolyglutamate synthase (folylpolyglutamate synthase)This gene encodes the folylpolyglutamate synthetase enzyme. This enzyme has a central role in establishing and maintaining both cytosolic and mitochondrial folylpolyglutamate concentrations and, therefore, is essential for folate homeostasis and the survival of proliferating cells. This enzyme catalyzes the ATP-dependent addition of glutamate moieties to folate and folate derivatives. While several transcript variants may exist for this gene, the full-length natures of only two have been biologically validated to date. These two variants encode distinct isoforms. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Folylpolyglutamate synthase. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | BovineBovine (81%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | FPGS antibody tetrahydrofolylpolyglutamate synthase antibody tetrahydrofolate synthase antibody folylpoly-gamma-glutamate synthetase antibody "folylpolyglutamate synthase mitochondrial antibody" folylpolyglutamate synthase antibody |
|---|---|
| Target | Folylpolyglutamate synthase |
| Molecular Weight(MW) | 65 kDa (note) (3) |
| Cellular Localization | Mitochondrion , Cytoplasm |
Application
Western Blot analysis of FPGS expression in transfected 293T cell line by FPGS polyclonal antibody. A: Non-transfected lysate. B: FPGS transfected lysate. 7.5% SDS PAGE diluted at 1:1000| Positive Control | Folylpolyglutamate synthase tansfected 293T cell |
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| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
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| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
